Journal: BMC Microbiology

Article Title: 6-thioguanine inhibits EV71 replication by reducing BIRC3-mediated autophagy

doi: 10.1186/s12866-025-03752-8

Figure Lengend Snippet:

Article Snippet: Mouse anti-GFP tag antibody , Proteintech (Wuhan, China) , 16825-1-AP.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Insulin Resistance Does Not Impair Mechanical Overload-Stimulated Glucose Uptake, but Does Alter the Metabolic Fate of Glucose in Mouse Muscle

doi: 10.3390/ijms21134715

Figure Lengend Snippet: Insulin resistance enhances overload-stimulated activation of the hexosamine biosynthetic pathway. After 12 weeks of high-fat diet (HFD) feeding, mice underwent unilateral synergist ablation surgery to induce overload (OVL) of the plantaris muscle for 5 days. ( A ) Muscles were excised and the protein content of glutamine fructose-6-phosphate transaminase 1 (GFPT1) determined by immunoblot (IB) analysis. The GFPT1 antibody was validated using mouse tibialis anterior muscle samples overexpressing GFPT1 (OE) or empty vector-transfected controls (-). ( B ) Immunoblots were also performed to assess total muscle O-GlcNAc-modified proteins. Data are mean ± standard deviation. Statistical analysis was performed using a 2-way ANOVA with Tukey’s post-hoc analysis. N = 8–10 muscles/group. p < 0.05 # vs. Sham (Sh); * vs. low-fat diet (LFD).

Article Snippet: Glucose-6-phosphate dehydrogenase (G6PD) and glutamine fructose-6-phosphate transaminase 1 (GFPT1) antibodies were validated using G6PDx or GFPT1 mouse muscle overexpression samples that were generated by transfecting the tibialis anterior muscles of male CD-1 mice (8 weeks old) with empty vector, untagged mouse G6PDx (cat#MC205271, OriGene Technologies, Rockville, MD, USA), or untagged mouse GFPT1 (cat#MC201036, OriGene Technologies, Rockville, MD, USA) vectors followed by standard in vivo muscle gene transfer/ electroporation procedures previously described by our lab [ , ].

Techniques: Activation Assay, Muscles, Western Blot, Plasmid Preparation, Transfection, Modification, Standard Deviation

Journal: International Journal of Molecular Sciences

Article Title: Insulin Resistance Does Not Impair Mechanical Overload-Stimulated Glucose Uptake, but Does Alter the Metabolic Fate of Glucose in Mouse Muscle

doi: 10.3390/ijms21134715

Figure Lengend Snippet: Immunoblotting conditions.

Article Snippet: Glucose-6-phosphate dehydrogenase (G6PD) and glutamine fructose-6-phosphate transaminase 1 (GFPT1) antibodies were validated using G6PDx or GFPT1 mouse muscle overexpression samples that were generated by transfecting the tibialis anterior muscles of male CD-1 mice (8 weeks old) with empty vector, untagged mouse G6PDx (cat#MC205271, OriGene Technologies, Rockville, MD, USA), or untagged mouse GFPT1 (cat#MC201036, OriGene Technologies, Rockville, MD, USA) vectors followed by standard in vivo muscle gene transfer/ electroporation procedures previously described by our lab [ , ].

Techniques: Western Blot, Blocking Assay